Journal: iScience

Article Title: Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells

doi: 10.1016/j.isci.2021.103605

Figure Lengend Snippet:

Article Snippet: Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] , Abcam , #ab220074.

Techniques: Produced, Recombinant, Enzyme-linked Immunosorbent Assay, Western Blot, CRISPR, Plasmid Preparation, Software

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Oral NaHCO 3 activates a splenic anti-inflammatory pathway; evidence cholinergic signals are transmitted via mesothelial cells

doi: 10.4049/jimmunol.1701605

Figure Lengend Snippet: Antibodies and Reagents utilized

Article Snippet: F4/80-A488, EMRI Bioss bs-7058R-A488 AE084243 100ul rabbit http://biossusa.com/store/datasheets/bs-7058R-A488 IL-10-Alexa647, CC320 Novus NB100-63026AF6477 1007-081415 250ul mouse ab http://www.novusbio.com/IL-10-Antibody-CC320_NB100-63026AF647.html CD44-FITC, OX-49 BD Bioscience 550974 4324539 .2ml mouse α rat Zimmerman MA, et. al ., Am J Renal Physiology 2015( 29 )& Tipton Am J PhysiolRegulIntegr Comp Physiol 2012( 28 ) CD69-PE, H1.2F3 eBioscience 12-0691-82 E01332-1634 .5ml α-mouse Yokoyama W, et. al ., JImmunol, 1988, 141( 2 ): 369-76(64).

Techniques: Cell Differentiation

Journal: Cells

Article Title: Blood and Sputum Eosinophils of COPD Patients Are Differently Polarized than in Asthma

doi: 10.3390/cells12121631

Figure Lengend Snippet: The comparison of CD125, CD193, CD62L, CD66b, CD14, CD11b expression on eosinophils in blood ( n = 10, 11, 14) and sputum ( n = 8, 9, 13) of control, asthma and COPD subjects. The data are presented as median (column) and individual data. Statistical significance between study groups was labeled as * for p -value ≤ 0.05; ** p -value ≤ 0.01; *** p -value ≤ 0.001.

Article Snippet: Cells were stained with antibodies against the surface binding molecules: CD45 (mouse anti-human APC-H7, cat. no. 641408), CD16 (mouse anti-human BV510, cat. no. 563830), CD125 (mouse anti-human BV421, cat. no. 743927), CD193 (mouse anti-human PE, cat. no. 558165), CD62L (mouse anti-human BV605, cat. no. 562719), CD66B (mouse anti-human PerCP-Cy5-5, cat. no. 562254), CD11B (rat anti-human APC, cat. no. 564985) and CD14 (mouse anti-human Alexa Fluor 488, cat. no. 557700) (BD Biosciences, San Jose, CA, USA) in BD Horizon Brilliant Stain Buffer (BD Biosciences, San Jose, CA, USA), and incubated for 20 min in the dark at room temperature.

Techniques: Expressing, Labeling

Journal: Cells

Article Title: Blood and Sputum Eosinophils of COPD Patients Are Differently Polarized than in Asthma

doi: 10.3390/cells12121631

Figure Lengend Snippet: The distribution of blood ( n = 10, 11, 14) ( A ) and sputum ( B ) ( n = 8, 9, 13) subpopulations of eosinophils defined by the expression of CD193 and CD125 in control, asthma and COPD groups. The data are presented as median (column), interquartile range (IQR) (whiskers) and individual data (points). Statistical significance between study groups was labeled as * for p -value ≤ 0.05; ** p -value ≤ 0.01; *** p -value ≤ 0.001; **** p -value ≤ 0.0001.

Article Snippet: Cells were stained with antibodies against the surface binding molecules: CD45 (mouse anti-human APC-H7, cat. no. 641408), CD16 (mouse anti-human BV510, cat. no. 563830), CD125 (mouse anti-human BV421, cat. no. 743927), CD193 (mouse anti-human PE, cat. no. 558165), CD62L (mouse anti-human BV605, cat. no. 562719), CD66B (mouse anti-human PerCP-Cy5-5, cat. no. 562254), CD11B (rat anti-human APC, cat. no. 564985) and CD14 (mouse anti-human Alexa Fluor 488, cat. no. 557700) (BD Biosciences, San Jose, CA, USA) in BD Horizon Brilliant Stain Buffer (BD Biosciences, San Jose, CA, USA), and incubated for 20 min in the dark at room temperature.

Techniques: Expressing, Labeling